INFLUENCE OF OXYGEN-CONTAINING SUBSTANCES ON VIABILITY OF CELL CULTURE IN EXPERIMENT

Abstract

The study aims to investigate the cytoprotective ability of oxygen-containing substances used in dermatology and cosmetology on the viability of cell culture during incubation in vitro. Material and methods. An experimental study to identify the cytoprotective ability of an oxygen-containing balm using the MTT assay is conducted. The key component of this assay is the use of tetrazolium salts in the form of 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide which are reduced to formazan in the presence of metabolically active cells. The transplantable cells HEK293 (human embryonic kidney, ATCC® CRL-1573 ™) are used as cell culture, and doxorubicin is used as a standard cytotoxic substance. Cell viability in the studied media is evaluated by measuring the optical density of the stained solutions by a spectrophotometer at a wavelength of 580 nm, followed by statistical processing of the results. Results. The ability of an oxygen-containing balm containing 20% of the NOVAFNEM-O2 oxygen complex at a dilution of 1:10 to prevent a decrease in the viability of isolated cells in the presence of the cytotoxic agent doxorubicin is established.