CORRECTION OF ANTINEOPLASTIC DRUGS INDUCED HEPATOTOXICITY USING IMMOBILIZED GRANULOCYTE-COLONY STIMULATING FACTOR

Abstract

The purpose is to examine the possibility of correcting antineoplastic drugs induced hepatotoxicity on the liver of experimental animals. To correct the hepatotoxic effects of the pharmarubicin and cisplatin antineoplastic drugs, the rats were injected with immobilized granulocyte-colony stimulating factor (immobilized G-CSF). The immobilized G-CSF was obtained by conjugation of non-glycosylated cytokine molecules with polyethylene glycol with a molecular mass of 1,500 G-CSF, and comparator drug Neupogen was administered to rats at a dose of 100 mcg/kg for 5 days, starting on the second day after a single injection of cytostatic drug. The results show the positive effect of immobilized G-CSF on the level of liver enzymes in experimental animals after cytostatic drug. The authors indicate normalization of liver enzyme activity in blood serum and a decreased intensity of inflammatory processes in the rats’ liver parenchyma caused by the administration of cytostatic drugs, as well as regenerative processes stimulation. Thus, the authors reveal that the immobilized G-CSF use after isplatin and pharmarubicin administration reduces the severity of the cytostatic drugs’ toxicity and damage on the liver of experimental animals.