SEVOFLURANE EFFECT ON HUMAN NEUTROPHILS

Abstract

The study aims to analyze the effect of sevoflurane on human neutrophils under normal and pathological conditions. Materials and methods. In the course of the research, the culture of neutrophils isolated from venous blood of donors was studied. The exposure of neutrophils was carried out with the minimal alveolar concentration of sevoflurane of 0.5, 1.0 and 1.5, evaluating the anesthetic effect on their activation. The apoptosis
intensity was assessed using annexin V and propidium iodide. Neutrophils activation with lipopolysaccharide and chemotaxis peptide N-formyl-methionine-leucine-phenylalanine was determined by the level of expression of neutrophil degranulation markers CD11b and CD66b, interleukin-1β, interleukin-6, interleukin-8 and phosphorylation of glycogen synthase kinase-3β. The statistical processing was carried out using math-and-stats methods of calculation of the basic measures of sampling distribution (arithmetic average, standard deviation, Student criterion, nonparametric methods), using Windows and Statistica 10.0 package. Results. Neutrophils
incubation with lipopolysaccharide (200 ng/ml) and N-formyl-methionine-leucine-phenylalanine (100 nM) statistically doubled the expression of CD11b and CD66b molecules, but the exposure to sevoflurane at the minimal alveolar concentration dose of 1.5 reduced the anti-inflammatory activation of neutrophils under the influence of lipopolysaccharide. Stimulation of neutrophils with lipopolysaccharide was accompanied with dephosphorylation of GSK-3β, and the exposure to the minimal alveolar concentration of sevoflurane of 1.5 contributed to its
phosphorylation. It is noted that phosphorylation of GSK-3β in neutrophils reduces the expression of CD11b and CD66b under the influence of sevoflurane.