EFFECT OF LITHIUM CHLORIDE ON ENDOTHELIAL CELLS IN PATIENTS WITH SEVERE CONCOMITANT INJURY COMPLICATED BY SYSTEMIC INFLAMMATORY RESPONSE
https://doi.org/10.34822/2304-9448-2021-1-87-91
Abstract
The study aims to research the protective effect of lithium chloride solution on endotheliocytes in cell culture under conditions of incubation with the blood serum of patients suffering from systemic inflammatory response
syndrome (SIRS) secondary to severe concomitant injury. Material and methods. We used serum of five patients with SIRS, which occurred in presence of severe concomitant injury, and blood serum of five healthy donors. Endothelial cells of the EA.hy926 line were incubated with the blood serum of healthy and sick patients. Lithium chloride was added to the cell samples at a concentration of 0.01, 0.1, 1, and 10 mmol/l. After incubation, the cells were removed with a Trypsin-Versene Solution, fixed with 70% ethanol, and stained with propidium iodide. Cells containing fragmented genomic DNA were analyzed using flow cytometry. Results. It was found that toxic serum inhibited phosphorylation of glycogen synthase kinase 3β (GSK-3β) in endotheliocytes, caused cleavage of Vascular endothelial (VE)-cadherin and a decrease in the amount of claudine and actin, which caused the destruction of
intercellular contacts of the endothelial monolayer and an increase in endotheliocyte apoptosis. Incubation of a monolayer of endothelial cells of the EA.hy926 line with lithium chloride at concentrations of 1 and 10 mmol/l
significantly prevented the disassembly of claudine, actin, and (VE)-cadherin, halved the intensity of endotheliocyte apoptosis, and stimulated the phosphorylation of GSK-3β.
About the Authors
O. A. Grebenchikov
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology,
Moscow
Russian Federation
Russian Federation
Doctor of Sciences (Medicine), Head, Organoprotection Laboratory
E-mail: oleg.grebenchikov@yandex.ru
M. D. Prokofyev
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow
Russian Federation
Russian Federation
Postgraduate, Department of Pathophysiology
E-mail: mdprokofyev@gmail.com
V. T. Dolgikh
V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow
Russian Federation
Russian Federation
Honoured Science Worker of the Russian Federation, Doctor of Sciences (Medicine), Professor, Chief Researcher, Laboratory of Clinical Pathophysiology of Critical States
E-mail: prof_dolgih@mail.ru
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Review
For citations:
Grebenchikov O.A., Prokofyev M.D., Dolgikh V.T. EFFECT OF LITHIUM CHLORIDE ON ENDOTHELIAL CELLS IN PATIENTS WITH SEVERE CONCOMITANT INJURY COMPLICATED BY SYSTEMIC INFLAMMATORY RESPONSE. Vestnik SurGU. Meditsina. 2021;(1 (47)):87-91. (In Russ.) https://doi.org/10.34822/2304-9448-2021-1-87-91
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