Functional disorders of biliary tract in children are frequently diagnosed. The aim of the research is to indicate the biomarkers of hypokinetic gallbladder dysfunction using ultrasound scanning and laboratory data on abdominal cavity organs in children. The research includes 58 children and teenagers. The authors distinguish 2 groups based exclusively on the differences in the gallbladder (IV) phase of the multifractional duodenal intubation. In the control group (29 people), the gallbladder phase does not exceed 30 minutes, and the secreted bile amount is 50 ml. In the study group (29 people), either the gallbladder phase exceeds 30 minutes, or the secreted bile amount surpasses 50 ml. The specified groups are comparable in age and sex. The paper includes laboratory studies and ultrasound scanning of abdominal cavity organs, particularly the gallbladder, with an assessment of its length, diameter, and volume. According to the biochemical blood test, the mean values of total protein, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) are statistically higher in children with gallbladder hypokinesia. However, the rate of abnormal test findings in the groups, as well as the results of the conducted ultrasound scanning of abdominal cavity organs in the children, show no significant differences. The bile length, diameter, and volume in children with the hypokinetic form of dyskinesia are 63.9 ± 1.81 mm; 20.7 ± 0.78 mm; 15.2 ± 1.38 ml, whereas in the control group the parameters are 65.1 ± 1.67 mm; 20.3 ± 0.83 mm; 14.9 ± 1.44 ml (р > 0.05). Therefore, ultrasound scanning demonstrates its lack of informative value in diagnosing the hypokinetic form of biliary dysfunction, especially gallbladder hypokinesia. Compared to a child's normal gallbladder functioning, the average ALT, AST, and total protein are significantly higher in children with the hypokinetic form of biliary dysfunction. Still, the aforementioned parameters do not surpass the standard values.